FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21457191
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG129900.061.03848694
CAAGTCG99000.030.28888794
GAAGTCG139350.025.70075294
TAAGTCG41250.025.40848494
TACGCTA8300.018.1204819
TATACGC8300.018.1204817
AAAGTCG52150.017.4841894
GTTTTTT273550.015.6179841
TTTTTTC354650.013.6103192
GCGAAAT25300.013.1897231
GAAAAAT205600.013.0987351
AAATCCA194250.012.8962674
GTTTAAT82200.012.6362521
GTTTTTC170150.012.5683211
GTTTTAC67800.012.5471961
CGAAAAT30850.012.4927051
GTCGATT21500.012.4604641
CTCGATT37050.012.3049931
AAAAATC212100.012.2984432
CGGATAT23500.012.21
Produced by FastQC (version 0.11.5)