FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21457191
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG153200.051.66318594
CAAGTCG116300.028.77385994
GAAGTCG136600.023.80966294
TAAGTCG42650.022.48065694
AAAGTCG62800.016.09076394
CGAAAAT34200.013.8801161
GTTTTTT270700.013.8725521
GCGAAAT24900.013.5903611
TACGCTA9350.013.0695189
CCCGAAC48000.012.8270831
CAATCGA21150.012.6666674
AAATCGA31850.012.5431714
CGAAAAC32600.012.3987731
CGCGAAC12150.012.3786011
GGAAAAT175750.012.2213361
CGCAAAT34450.012.1422341
CGAATCG14100.011.9999993
AAATCCA191050.011.9560324
CTCGATT35500.011.9154921
CTCAGAT176350.011.8865891
Produced by FastQC (version 0.11.5)