FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22436182
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103950.051.18229394
CAAGTCG84900.028.3992994
TAAGTCG29700.023.73737394
GAAGTCG110950.017.9188894
GCGGTAT24800.014.7822571
AAAGTCG46300.014.1101594
GTTCGAT31800.012.4150931
AATCGTA20450.012.1809285
GGCGAAT58500.011.2478621
GTCCGAT15550.011.1832791
TTCGATT34850.010.9239592
GTTTATT98150.010.4391231
TTAGCGA20750.010.4192778
CGTATCG17200.010.383723
CGAATCG17150.010.1399413
CGGTATA12550.010.1115532
CGGGAAT52150.09.913711
TATACGG15950.09.87147334-35
GTCGGTT48150.09.7611631
CGCGTTT14000.09.41
Produced by FastQC (version 0.11.5)