FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22436182
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130100.041.07532594
CAAGTCG103700.025.6075294
TAAGTCG28750.018.47304394
GAAGTCG116550.016.61432694
AAAGTCG49050.013.79816494
GCGGTAT25450.012.5579561
GTCCGAT15150.011.1683161
GACGCTA23650.010.9302324
GTTCGAT32500.010.8461531
GTTTATT106700.010.6597931
GCCGGTT58950.010.6039011
CGCGTGT23950.010.4008341
CGCGTAA8251.1204975E-910.2545451
TTAGCGA22150.09.9729128
ACGCTAC25700.09.8754855
CGCGAAT4409.998186E-49.6136361
GTCGTTT19600.09.5918371
GGGAAAT144700.09.5494121
TATACGG16250.09.54461534-35
TTCGATT33750.09.4696292
Produced by FastQC (version 0.11.5)