FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23264190
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG88200.045.98752694
CAAGTCG69500.023.2633194
TAAGTCG30150.021.98009994
GAAGTCG106950.016.25993394
GTTTTTT355750.014.7572741
TTTTTTC488000.013.98442652
GTTTTAC86350.013.3896921
TATACGC9300.013.1397847
CGAAGAT84100.012.5743161
CGAAAAT41200.012.5485431
CGGAAAT49100.012.4439911
CGCAAAT29500.012.4271181
TTTTTTG524050.012.2959642
GCGAAAT28300.012.2897521
TTTTTTA513150.012.218262
GAAAAAT266400.012.0675671
CGAAAAC36550.011.9589591
AAAGTCG50000.011.7594
GTCGGTT46100.011.6225591
GTTTAAT113400.011.6049381
Produced by FastQC (version 0.11.5)