FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23114092
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG90650.045.52234694
CAAGTCG75150.020.82634794
TAAGTCG32150.019.88180594
GAAGTCG103550.016.29454494
GTTTTTT326350.015.2802221
CGAATCG16150.015.1331273
CGGATAT25800.015.1201561
TTTTTTC464850.014.6808652
CTCGATT39150.013.5657741
CGAAAAC35550.013.2208171
AAATCGA35300.013.1813044
CGAAAAT41700.012.9616311
TACGCTA7501.8189894E-1212.5333349
TTTTTTG513900.012.520532
AAAGTCG50250.012.34626994
TTTTTTA482650.012.1042172
CGCAAAT29850.011.9664991
GTTTTAC83800.011.9463011
GGAAAAT197850.011.9251971
CGAGAAC69050.011.9116581
Produced by FastQC (version 0.11.5)