FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22170187
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126000.055.5047694
CAAGTCG97550.029.9200494
TAAGTCG36700.026.50953794
GAAGTCG138850.023.08534494
AAAGTCG51000.016.31176494
GTTTTTT298450.013.9370081
CGAAAAT35950.012.0278161
TTTTTTC387900.011.9590092
GTCGAAT14450.011.3840831
GTTTAAT93500.011.3101591
GTTTTAT153950.011.2348161
GGAAAAT181900.011.0329851
CGGAAAT46500.011.0172031
TCGGATC39800.010.982412
CGCATAT11300.010.8141591
GTTTTCT223600.010.8041141
CGGATAT24450.010.7648261
CGAAAAC35050.010.7275311
GCGAAAT29900.010.5317721
CGAAGAT84750.010.4814161
Produced by FastQC (version 0.11.5)