FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22099051
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114050.054.52082494
CAAGTCG86700.026.29181194
TAAGTCG32000.024.3812594
GAAGTCG128450.019.83184294
AAAGTCG51050.016.94025694
AATCGGG45750.012.8415315
GTCGGTT46550.012.8227721
CAATCGG30650.012.2675374
TACGCTA7803.6379788E-1212.0512839
CGAAAAT35300.011.8498581
TTAGCGA23050.011.8264667
CGGAAAT46450.011.5349851
GTCGAGT20200.011.40099051
AACGTCG28100.011.3736659
GTTTTTT272450.011.3165721
TATACGC7101.029548E-911.2535227
TAGCGAG25350.010.7534528
AAATCGA33450.010.6786264
AGTAACG29100.010.6597956
GTCGTAT11500.010.6260861
Produced by FastQC (version 0.11.5)