FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22099051
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136150.042.28791894
CAAGTCG98550.025.70573494
TAAGTCG33100.021.4410994
GAAGTCG126250.017.57148694
GTCGGTT43050.013.2102221
AATCGGG37950.012.0131765
GTCGTTT21050.011.833731
AAAGTCG57050.011.78089494
GTCGAAT12850.011.7042811
CTCGGAT65150.011.3983131
CGAAGAT78350.011.2776011
CGAAAAT44450.010.9966261
CGCGGAT26900.010.8327141
CGCAAAC42750.010.6643291
GTCCGAT12800.010.64843751
CGCGAAC16900.010.5680481
CGCAATT22700.010.5594711
GTTTTTT267950.010.5418921
CGCAAAT30850.010.51215651
GGAAAAT172050.010.4626571
Produced by FastQC (version 0.11.5)