FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21310929
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124500.054.39919394
CAAGTCG93500.029.70802194
TAAGTCG37550.024.65778794
GAAGTCG141250.023.45840894
AAAGTCG44950.016.52057694
CGCAAAT30200.016.4966891
TATACGC8400.016.226197
GTTTTTT262200.015.7383681
TACGCTA9100.015.4945069
ATATACG11500.014.3043476
TTTTTTC333850.013.7966162
AAAAATC186600.013.7775982
CGCGTAT4554.8432412E-813.4285721
AAATCCA177550.013.0239374
GCGAAAT28900.013.0103811
GTTTAAT75650.012.9226691
AATCCAC102400.012.8974615
CGAAAAT34450.012.8243831
GCCGAAT48100.012.604991
GAAAAAT194500.012.5897171
Produced by FastQC (version 0.11.5)