FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23144918
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86850.050.16580294
CAAGTCG71000.024.36056594
TAAGTCG28200.023.33333494
GAAGTCG110150.017.87834794
GTTCGAT28000.017.1214281
CGCGTAT4601.2732926E-1116.3478281
TTCGATT32850.014.0213092
GTCCGAT14600.013.1986291
CTCGGTT38650.012.4036231
TCGGATT26750.012.1233642
TATACGG17500.012.08571334-35
AAAGTCG41950.011.9880894
CGTAACG5202.7620263E-711.753
TTAGCGA22100.011.2714948
GTCGAAT13650.011.0183141
GTCGGTT50400.010.8174611
TAGCGAG26850.010.6778399
GTCGTTT24050.010.5530151
CCCGTAT28700.010.4808361
GTCGGAA35200.010.4147721
Produced by FastQC (version 0.11.5)