FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23144918
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113450.038.2379994
TAAGTCG31000.021.98387194
CAAGTCG89150.021.40437394
GAAGTCG111050.016.29446294
GTTCGAT28450.015.1985931
TTCGATT31250.012.7839992
TATACGG18000.011.7534-35
CTCGTTT40400.011.6336641
CCCGTAT25400.011.2874011
GTCGTTT25350.011.124261
GTCCGAT14150.010.6289761
TCGATTC35400.010.62146953
GTCGGTT48100.010.5530151
GTCGAAT15800.010.4113921
CGCGTTT14250.010.2245621
TTAGCGA20800.010.1682698
GTATACG18300.010.14480934-35
CTCGGAT59500.010.1109251
CGCATTT37550.010.0133151
CGCGTAT5204.1568976E-59.9423071
Produced by FastQC (version 0.11.5)