FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21589459
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107950.059.69152594
CAAGTCG87300.030.84879994
TAAGTCG34100.025.77419594
GAAGTCG129850.023.20138794
AAAGTCG47150.020.2354294
TATACGC7400.015.8783797
GTTTTTT293550.014.7940741
TTTTTTC381200.014.64742852
TACGCTA8400.013.9880969
GCGAAAT28500.013.3578961
ATATACG11200.013.0089296
TTTTTTG413550.012.9902082
TTAGCGA22650.012.6578387
CGAATCG14950.012.5752523
CGAAAAT35050.012.3366631
CGCAAAT28400.012.0809861
GAAAAAT212050.012.035371
CGGTATA13000.011.930772
GGAAAAT158500.011.7722421
AAAAATC204600.011.7385152
Produced by FastQC (version 0.11.5)