FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21589459
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135300.047.62528294
CAAGTCG103550.028.86721694
TAAGTCG33150.025.94570294
GAAGTCG125000.021.469694
AAAGTCG59400.016.69528894
CGAATCG16250.013.3046163
GCGAAAT25850.013.0909091
CGAAAAT42500.012.60705951
GTTTTTT282500.012.5444251
TATACGC7701.8189894E-1212.2077937
CGGAAAT44300.012.2009041
CGAAAAC34750.011.7669071
GGAAAAT155050.011.6098031
AAAAATC192200.011.1997932
CGCGAAC17850.011.0588241
AAATCCA188250.010.8855264
CATATCG18150.010.8760343
CGCGAGT29350.010.7291321
GCCGAAT48800.010.6905751
CTCGATT37000.010.5432431
Produced by FastQC (version 0.11.5)