FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23796777
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105400.048.6053194
CAAGTCG79750.025.69529594
TAAGTCG30350.024.62273494
GAAGTCG129400.017.72488494
CGAAAAT34450.014.1886791
GTCGGTT49600.013.9294351
AAAGTCG49850.012.16248794
GTTTTTT312650.011.3798171
AATCGGG43150.011.2190045
CGCGAAC11300.010.8141591
CGAAGAT87000.010.7505741
GGAAAAT185750.010.6777931
GTCAATT61450.010.5549221
GCCGAAT46450.010.5231431
GTCGATT23400.010.4444451
GTTTTAC76000.010.2657891
GTTTTAT154600.010.2451481
GTTTAAT98900.010.217391
CGAATCG16600.010.1927713
CGCAAAT33900.09.98231
Produced by FastQC (version 0.11.5)