FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23796777
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137450.037.92142594
CAAGTCG107400.022.75605294
TAAGTCG34350.021.34497694
GAAGTCG125000.015.679294
CGGAAAT46200.013.9372281
CGAAAAT44300.013.7923241
CGCGTAT6309.094947E-1213.4285711
GTCGGTT44200.012.2285061
TATACGC7501.6370905E-1111.9066677
AAAGTCG58100.011.567986594
CTCGGTT43300.011.1801381
CGAAGAT83900.011.0357561
CTTTAAT107200.010.960821
CGCAAAT36750.010.87074851
TACGCTA8752.5465852E-1110.7428579
CGTATCG18450.010.6991853
CGAATAT14650.010.587031
GGAAAAC162550.010.5247621
GGAAAAT184500.010.3680211
CGCGTTT16350.010.3486231
Produced by FastQC (version 0.11.5)