FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22248694
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126250.052.78891494
CAAGTCG94300.025.6182494
TAAGTCG30850.025.59481494
GAAGTCG140450.021.2830294
TACGCTA7850.016.1656069
AAAGTCG50850.015.71288294
GTTTTTT271650.015.2081741
TTTTTTC354000.015.148872
TTTTTTG395000.013.3146842
CGAAAAT33050.013.0832071
TATACGC9700.013.0824757
CGCGAAT7600.012.9868431
GTTTAAT80700.012.6964071
ATATACG11350.012.4229086
CGAATCG13700.012.00733
CGCAATT19850.011.8387921
GTTTTTC152550.011.8000651
GTTTTAC59600.011.7500011
CGGAAAT45350.011.7111371
CGAAAAC32200.011.6770191
Produced by FastQC (version 0.11.5)