FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22248694
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG148050.045.07936594
TAAGTCG32650.025.19142794
CAAGTCG108600.024.14917294
GAAGTCG134000.021.36044794
GTTTTTT265200.014.8337111
TATACGC9900.014.7171717
CGCGAAT8200.013.7560981
AAAGTCG56650.013.27449394
CGCAATT19550.013.2225071
GTTTAAT77650.013.19510651
CGAAAAT37050.012.6855611
CGAAAAC35050.012.4707561
CGCGTAT4701.0418298E-612.01
TCGAATC17150.011.7842582
CGGAAAT44450.011.7367841
CGCAAAC42600.011.6948371
AAATCGA30150.011.5356564
TACGCTA9900.011.3939399
CGCGTTT15400.011.2922091
GGAAAAT155150.011.2690951
Produced by FastQC (version 0.11.5)