FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21848195
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139200.060.640894
CAAGTCG109750.032.24692594
TAAGTCG38800.029.07216394
GAAGTCG143350.025.40983494
AAAGTCG55850.019.18710794
CGAAAAT34450.013.779391
GTCGATT20650.013.6561741
GTTTTTT287150.013.0942011
CGCGAAT9550.012.795811
GCGAAAT27250.012.7633021
TTTTTTC365900.012.7422772
GAAAAAT211550.012.1748991
GTTTAAT87300.011.8980521
AAAAATC204800.011.7499992
GGAAAAT165750.011.3140281
TTTTTTG403950.011.2860492
CGCATAT7201.2969394E-911.0972211
CGAAAAC32100.010.9813081
CGAAGAT74750.010.9404671
AATCCAC110950.010.9292475
Produced by FastQC (version 0.11.5)