FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21848195
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG166950.050.6738594
CAAGTCG125850.029.12991794
TAAGTCG37400.026.26470694
GAAGTCG143500.024.9574994
AAAGTCG63550.017.82376194
CGCGAAT8150.015.5705511
GTTTTTT285800.013.4356181
GCGAAAT25700.012.4357981
CGAAAAT36800.012.3885861
AAAAATC196800.011.5828252
CGAAAAC34300.011.5102031
GTCGGAT39650.011.49810894
AAATCGA32700.011.2110094
GTTTTCT217300.011.0957191
GAAAAAT204250.011.0222761
AAATCCA201750.010.9491934
CGGAAAT44300.010.821671
GGAAAAT161500.010.7095971
AATCCAC110950.010.6750785
CTCGGAT58750.010.5599991
Produced by FastQC (version 0.11.5)