FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22134852
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136350.045.0869194
CAAGTCG104550.024.94978794
TAAGTCG35100.022.4957394
GAAGTCG123700.020.85933994
AAAGTCG55100.013.73321394
CGGAAAT44400.013.4436951
CGCGAAT9800.013.4285731
GTTTTTT307250.012.6812051
AAATCGA34100.012.1290334
GCGAAAT25650.011.9103321
CGCAAAC43350.011.3840841
CGAAAAT45100.011.1507771
GGAAAAC157150.011.1256761
GTTTAAT92150.011.0678241
GGAAAAT176350.010.9004821
AATCCAA113100.010.8877115
GGCGAAT34900.010.773641
GCCGATT21500.010.7116281
TCGGATT26850.010.677842
AATCGAG37550.010.6391495
Produced by FastQC (version 0.11.5)