FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23116612
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96250.047.317494
CAAGTCG80600.025.59925794
TAAGTCG27350.020.2778894
GAAGTCG112250.018.2556894
AAAGTCG43050.012.22764294
GTTTATT102100.011.5543581
GTTCGAT29000.011.1827581
TCGGATT27250.010.8660552
TTCGATT32250.010.7844962
GTCGAAA32650.010.5084221
GGGAAAT165050.010.2799161
CGCGTAT5506.8703393E-610.2545451
GTCGAAT13300.010.248121
CTCGGTT39500.010.2329121
CTCGGAT63400.010.2302841
GTCGGAT29700.010.1279471
CGGGAAT55750.010.1165921
CGCGGAT24650.09.7241381
TATACGG15750.09.69841334-35
CTTTATT165500.09.2296071
Produced by FastQC (version 0.11.5)