FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23116612
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120800.037.62334494
TAAGTCG28550.025.35201594
CAAGTCG86350.022.53387394
GAAGTCG121900.016.07793294
CGCGTAA8850.012.7457631
AAAGTCG52100.011.18618194
GTTTATT109600.011.06386851
GTTCGAT29450.010.8522921
GTCGAAT14300.010.8461541
CTCGGTT38750.010.7948381
TCGGATA18800.010.752
GTCCGAT15750.010.7428581
CTCGGAT63300.010.6919431
CGTATCG18250.010.5589043
GTCGTTT24000.09.98751
GTCGTGT37850.09.933951
GTACGAT14800.09.8445949
TACCGTA17050.09.6480949
GTCGGTT52250.09.624881
CGGGAAT56300.09.5168731
Produced by FastQC (version 0.11.5)