FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21891741
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95500.047.6397994
CAAGTCG74400.025.45833494
GAAGTCG110650.018.09489494
TAAGTCG29850.017.79229594
GTTTTTT273850.016.0299431
TTTTTTC375600.015.9544732
AAAGTCG46000.015.42826194
CGAATCG18450.015.2845533
TTTTTTG408200.014.6687912
CTCGATT47100.014.5690021
GTTTAAT91850.013.5601531
GCGAAAT28900.013.173011
TTTTTTA387900.013.1343142
GAAAAAT230650.012.4504661
GTTTTAC68900.012.4150951
AATCGTA22750.012.3956045
AAATCCA196500.012.3659044
AATCCAC118950.012.20933155
CGAAAAC34350.012.0407561
AAAAATC213600.012.0140452
Produced by FastQC (version 0.11.5)