FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21891741
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124250.036.8434694
CAAGTCG91500.022.54972894
TAAGTCG30500.018.95409894
GAAGTCG110850.017.38385294
TACGCTA9300.015.6666689
CGAATCG17350.015.1700293
AATCGTA19600.015.1071435
CGCAAAC39100.015.0255761
GTTTTTT298850.014.6889741
CGAAAAC36250.014.6510341
AAATCGA30900.013.9935274
CTCGATT47500.013.9515781
TATACGC10350.013.1690827
AAATCCA187850.013.085444
AAAAATC200600.012.6051852
CGGAAAT45150.012.5957921
ATATACG12750.012.5333346
CGTAATG18250.012.3616448
GTTTTAC71500.012.2265741
AATCCAC109400.012.2010975
Produced by FastQC (version 0.11.5)