FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22286788
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108900.052.9990894
CAAGTCG83100.027.5439294
TAAGTCG37250.021.0711494
GAAGTCG124400.019.0795894
AAAGTCG50300.015.04373694
CGAAAAT32100.014.0560741
GGAAAAT182200.012.1498351
GTTTTAT147650.011.8096841
CGAAGAT85850.011.6062891
GTTTTTT288100.011.5827831
GTTTATT102350.011.3883721
GTCGGTT47150.011.2640511
CGGAAAT44800.011.2254461
CTTTAAT105400.011.0588231
CGCGTAT5102.7848237E-611.0588231
CGCAATT22050.010.6575971
CGCGGAT23400.010.6452981
CGACGAT28400.010.5915481
AAATCGA33300.010.5855864
TCGGATC43450.010.4925192
Produced by FastQC (version 0.11.5)