FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21977379
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83100.041.51383694
CAAGTCG67450.020.62564794
TAAGTCG27600.018.73188494
CGCAAAT27000.015.4925931
CGGATAT23750.015.2378941
GTTTTTT298350.014.949891
TTTTTTC422000.014.924172
AAATCGA33850.014.7178734
CGCGAAT15050.014.3654481
CGAAAAT36150.014.301521
CGAATCG17150.014.2507293
GAAGTCG104350.014.1878394
TTTTTTG464850.013.7708932
GCGAAAT24900.012.83534051
CGAAAAC32350.012.7851621
TTTTTTA421250.012.6077152
CTCGATT39950.012.352941
AAATCCA199200.012.1982934
GAAAAAT218750.012.0321
GTTTTTC175050.011.92116451
Produced by FastQC (version 0.11.5)