FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21218372
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG51650.017.74443294
GTTTTTT326950.017.2215921
CGAAAAC31850.015.1993721
CGAAAAT34650.014.649351
TTTTTTC391600.014.1744122
GCGAAAT30650.013.64763451
AAAAATC207750.013.121542
AAATCGA29300.012.9931744
GTTTAAT93300.012.5434071
GAAAAAT210450.012.4395331
AATCCAC111750.012.36515
AATCCAA96400.012.2863065
GCCGATT21500.012.2418591
CGCAAAT34750.012.1726611
AAATCCA183900.011.9864044
GTTTTAC63650.011.9622931
TTTTTTG452900.011.8719362
TACGCTA7953.6379788E-1211.8238989
TATACGC7953.6379788E-1211.8238987
CGCTAAT9550.011.8115181
Produced by FastQC (version 0.11.5)