FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21218372
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT297500.015.308571
CGCAAAC39850.013.917191
TACGCTA8450.013.9053259
AAATCGA28500.013.6877194
CGCAATT21300.013.680751
CGAAAAC31750.013.6188971
GCGAAAT27100.013.3542421
TATACGC7850.013.1719747
CTCGATT35450.012.8603661
CGCAAAT34350.012.4512361
TCGATTG23150.012.3844482
GTTTAAT83500.012.3269451
CGCGGAT21300.012.1361491
GGAAAAT154100.012.1083711
AAGTCGG76800.012.05598994
CTCGAAT34000.012.026471
CGAATTG22500.011.9066663
GTTTTTC154200.011.8261991
CTTTGAT160400.011.751
AAATCCA192500.011.6950654
Produced by FastQC (version 0.11.5)