FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259295
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG176450.052.3139794
CAAGTCG139750.030.30197194
TAAGTCG39300.024.99491594
GAAGTCG153500.024.954494
AAAGTCG66000.017.3757694
CGCGAAT11900.016.5882361
GTTTTTT262500.014.807241
GCGAAAT29450.013.8845511
AATCCAC117250.013.7893395
AAATCCA196850.013.5138444
CGAAAAC35500.013.5042271
CGCAAAT31500.013.27936651
AAAAATC192100.013.1874022
CGCAAAC43850.012.96921351
CGGAAAT48050.012.91155051
GCCGAAT50150.012.6520441
CGCGTAT4557.0384885E-712.3956051
CTCGGAT67900.012.3902811
CTCGATT39200.012.2295921
GAAAAAT194950.012.2231351
Produced by FastQC (version 0.11.5)