FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24369959
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139550.057.96273494
CAAGTCG111300.030.69990794
TAAGTCG40850.026.117594
GAAGTCG153750.022.162694
AAAGTCG58700.018.5758194
TATACGC8900.016.8988767
GTTTTTT306700.014.3436571
TTTTTTC388250.014.2725052
TACGCTA9500.013.85263259
ATATACG12300.013.7560966
CGCGAAT23000.013.2826081
TTTTTTG405600.013.0941812
CGAAAAT38150.012.935781
GAAAAAT228750.012.8620761
AAAAATC219400.012.4676392
GTTTAAT100750.011.89578151
CGAATTG32500.011.858463
CGCGAAC13200.011.7499991
CGGAAAT51350.011.7156761
AATCCAC129800.011.6956855
Produced by FastQC (version 0.11.5)