FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22591912
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG111050.047.52903794
CAAGTCG87000.026.68735794
TAAGTCG30750.021.39837394
GAAGTCG128700.018.66122894
TATACGC9850.017.1776667
TACGCTA10900.016.385329
ATATACG14800.015.2432436
GTCGGTT40750.013.3791411
GCGGTAT31000.013.1903231
AAAGTCG50650.013.176702594
CGCGTAT5452.8139766E-912.935781
CGCAAAT34150.012.52415851
AATCGGG42500.012.496475
CGCGAAC17500.012.3542861
GTTTTTT274100.012.2772721
CGAAAAT34600.012.2254331
GTTTAAT90450.012.0033171
GCGGAAT42200.011.9170621
GGAAAAT168550.011.7953131
GAAAAAT214200.011.3879551
Produced by FastQC (version 0.11.5)