FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22143381
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG161700.050.9820694
CAAGTCG118750.031.1090594
TAAGTCG38550.026.57846694
GAAGTCG148500.021.49023494
AAAGTCG66600.016.2312394
GCGAAAT29600.016.037161
GTTTTTT274650.015.5212081
CGCGAAT12150.013.9259241
GCCGAAT53850.013.7028781
CGAATCG19200.012.9739583
CGCAAAC46850.012.84098151
AAAAATC193300.012.813762
CCGAATC71100.012.7580872
AAATCCA192400.012.727134
CGGAAAT47600.012.6386551
CGCGTTT15600.012.3525631
CTCGATT41300.012.1767551
CGTGATA22050.012.1496599
CGAAAAT45800.012.109171
GAAAAAT199550.012.1062391
Produced by FastQC (version 0.11.5)