FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22195565
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130650.061.33562594
CAAGTCG101150.030.5279394
TAAGTCG37500.026.31999894
GAAGTCG146900.024.73178994
AAAGTCG51950.017.91337894
GTTTTTT283300.016.7560881
GCGAAAT30050.015.9534091
GTCGAAT14600.015.77397251
TTTTTTC364900.015.1342832
CGAAAAC35600.014.5224711
GAAAAAT211650.013.4349161
TCGAATC19300.013.3937822
TACGCTA7401.8189894E-1212.70270259
AAATCCA185200.012.6889844
GTTTTAT157550.012.6486831
AATCCAC114150.012.6403855
GCGGAAT42950.012.58441
TTTTTTG398500.012.5372642
GGAAATT94400.012.1483041
CGGATAT22450.012.1425381
Produced by FastQC (version 0.11.5)