FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22195565
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG157400.050.64294494
CAAGTCG122450.029.01755794
TAAGTCG35750.023.9272794
GAAGTCG139800.021.98712394
AAAGTCG62550.016.98161394
GTTTTTT277250.014.443281
CTTTAAC74300.014.0430681
GCGAAAT27850.014.007181
CGAATCG17500.013.428573
CGAAAAC36700.013.3188011
AAATCGA31100.012.8456584
CTCGATT36300.012.5592281
AAATCCA196600.012.5269584
AATCCAC115400.012.3812825
GCCGAAT45900.012.2875811
CGCAAAC44100.012.2562351
CGCAAAT31000.012.1290321
AAAAATC198800.011.9391352
CTTAGAT51450.011.7842551
CGCAATT20000.011.751
Produced by FastQC (version 0.11.5)