FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22644391
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131850.059.2089594
CAAGTCG100450.029.05624694
TAAGTCG35850.028.84239694
GAAGTCG142550.024.2006394
GTTTTTT311400.018.00611
AAAGTCG50650.016.14610194
TTTTTTC390100.016.0843352
CGAAAAT42300.015.7777761
TTTTTTG424950.014.0463572
GTTTAAT99800.013.9869721
TATACGC9050.013.5027627
GAAAAAT220450.013.4316181
CGAAAAC34350.013.2721971
AAAAATC212300.013.2388122
CGTGATA22100.013.1855199
GTTTTCT217250.013.0020711
CGCAAAC45250.012.8795571
GTTTTAC63500.012.8787391
AATCCAC125000.012.7463995
CTCGATT44000.012.7113631
Produced by FastQC (version 0.11.5)