FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22718298
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112650.058.07723694
CAAGTCG95050.029.569794
TAAGTCG32750.027.12366794
GAAGTCG123000.019.6024494
AAAGTCG44700.016.40268594
GTTCGAT33450.014.1913311
CGCGTAA9350.013.0695191
TATACGG18950.012.64907734-35
GCGGTAT26550.012.5687391
TTCGATT36300.012.5592282
CGCGTAT3801.891268E-512.3684221
CGGTATA13400.011.9253742
GTCGGAT30400.011.9046061
GTCCGAT13700.011.3211691
GTCGGAA39450.010.9607111
CGTCGGA28600.010.84615494
CGTATCG19600.010.7908173
GTTTATT97250.010.7773781
GTATACG19350.010.32299834-35
TTAGCGA27450.010.2732248
Produced by FastQC (version 0.11.5)