FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22252548
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139250.048.33321494
CAAGTCG115050.029.74011294
TAAGTCG33300.025.9699794
GAAGTCG122400.019.92892394
AAAGTCG53400.015.84269794
GTCCGAT15400.012.8181821
CGTATCG17250.011.9884063
TTAGCGA17600.011.4829558
AATCGTA22100.010.6334855
CGCGTAT4501.0605568E-410.4444441
ATCGTAT19900.010.3919596
GTCGGAT34200.010.30701794
TCGGATT25450.010.1571712
GGGAAAT147850.010.1406831
CGGGAAT52100.010.0134351
CGCGGAT25100.09.9243021
TAGCGAG24450.09.6114519
GTCGTGT32100.09.5171351
GCGGTAT22650.09.1302431
GGCGGAT84500.09.1218941
Produced by FastQC (version 0.11.5)