FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21831974
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104450.051.38726494
CAAGTCG79100.026.1441294
TAAGTCG31250.023.61279994
GAAGTCG119950.019.3955894
TATACGC10750.017.0511637
GTTTTTT270150.016.8758091
TACGCTA9700.016.4742269
TTTTTTC351850.015.7757572
CGAAAAT38300.015.7075721
AAAAATC212050.014.3404852
CGCGAAT12550.014.2310761
TTTTTTG394050.014.145922
ATATACG12850.013.8988326
CTCGATT57750.013.5913421
GAAAAAT214850.013.34419251
AAAGTCG46500.013.24085994
GTTTTAC61400.013.1661231
CGCAAAT30750.013.1447151
AAATCCA195800.013.1302354
GCGAAAT30800.013.1233761
Produced by FastQC (version 0.11.5)