FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22365000
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95150.048.160894
CAAGTCG75500.026.70596194
TAAGTCG30150.026.34494294
GAAGTCG120950.018.96320794
AAAGTCG44400.016.0900994
GTTTTTT283850.014.8856791
TTTTTTC368000.014.8663052
CGCGAAT12750.014.7450981
CGAAAAT37050.012.5587041
TTTTTTG401400.012.528652
GTTTAAT88350.012.0226371
TTTTTTA370500.011.9624832
GAAAAAT209050.011.9607751
GCGAAAT33550.011.90761
GGAAAAT163450.011.8470481
AAAAATC206300.011.8012612
AAATCCA198500.011.7914354
CGAAAAC33250.011.732331
GTTTTCT208800.011.6599631
AATCCAA98400.011.4634145
Produced by FastQC (version 0.11.5)