FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23150951
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG74450.041.4761694
CAAGTCG63300.020.78988894
TAAGTCG23000.020.43478294
GAAGTCG103600.013.24710594
AATCGTA25400.012.7677165
GTCGTTT20500.011.6926821
GGCGATT36150.011.571231
GTCGAAA34550.011.4269161
ATCGTAT20200.011.400996
CGCTTAA32800.011.39176844-45
GTCGGGT42950.011.1618161
CTCGGTT39000.010.4846141
GGGAAAT172450.010.3838791
GGCGAAT56700.010.3615511
CGCGAAT5456.1614464E-610.3486231
GTTCGAT26950.010.2894251
GTCATTT154800.010.2622731
GTCGGTT52500.010.2057141
GGCGTTT48950.010.0817161
GTCCGAT12600.010.0714281
Produced by FastQC (version 0.11.5)