FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22933317
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG89850.049.1185394
CAAGTCG68500.028.47445194
TAAGTCG32300.021.68111494
GAAGTCG106750.017.91943794
CGCGTAA9150.012.3278691
CGACCTA13450.012.2304844
AAAGTCG47850.012.08150594
CGCTTAA38700.011.78036244-45
GTTCGAT32550.011.4070661
CGTTCGA16600.011.3253017
AATCGTA29050.011.3253015
ATCGTAT22550.011.254996
GCGGTAT22400.011.1205361
CGCGTTT13750.010.9381821
TATACGG17150.09.86588934-35
GTCGTTT23100.09.7662331
CGGGGTT88250.09.6929181
GTCCGAT16050.09.6635511
CTCGGTT38050.09.6346921
TTCGATT37650.09.4873842
Produced by FastQC (version 0.11.5)