FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23385917
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109200.050.35714394
CAAGTCG88350.026.43916194
TAAGTCG34050.021.94713694
GAAGTCG127600.018.711694
CGAAAAT38500.014.0389611
TATACGC8950.013.1284927
AAAGTCG49000.013.04489794
GTCGAAT14750.012.7457631
CGAATCG16100.012.5527953
CGGAAAT45750.012.3278691
TACGCTA8100.012.18518459
GGAAAAT179250.011.85160351
ATATACG11750.011.66
GTTTATT106250.011.5896471
AATCGTA21200.011.5283025
CGAAGAT90600.011.2052971
GTTTTAC66650.011.0007511
GTTTTTT301200.010.9853921
CTCAGAT180450.010.9653641
GTCGGTT49450.010.7401421
Produced by FastQC (version 0.11.5)