FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23031879
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134700.042.56867294
CAAGTCG99650.024.90316294
TAAGTCG32650.022.02450494
GAAGTCG130800.017.75076794
CGCGTAA7900.012.4936711
GTCGGTT43500.012.3172421
TCGGATT26750.012.1233642
GTTCGAT32450.011.5870581
CGCGTAT5505.708407E-711.1090921
CTCGGTT40450.010.8059341
GTCGTTT19650.010.5241731
AAAGTCG52200.010.35440694
TACCGTA16950.010.2595869
CGGGAAT57600.09.9548621
CGCGGAT27400.09.9489051
GGCGAAT43600.09.9174311
CGAAGAT84900.09.9093061
CGGAAAT43500.09.8321851
CGTATCG18650.09.8284193
GTCCGAT14950.09.745821
Produced by FastQC (version 0.11.5)