FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22509503
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116450.059.97595694
CAAGTCG87800.030.24487594
TAAGTCG35850.027.53138294
GAAGTCG135700.023.205694
CGCGTAT4400.020.2954541
AAAGTCG47800.016.91213494
CGAAAAT34900.014.1404011
GTTTTTT313650.014.100751
TTTTTTC392050.013.1870942
CGCAATT19800.012.8181831
CTCGATT35600.012.6741561
GTTTAAT93850.012.4198191
TTTTTTG434450.012.1164682
CGCGAAC18300.012.0710381
TCGATTG22900.011.9039312
AAATCCA198350.011.8951344
AATCCAC120700.011.7986755
GGAAAAT176900.011.5308081
CGGAAAT39150.011.5249041
GTTTTAC67200.011.4702391
Produced by FastQC (version 0.11.5)