FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22509503
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG141800.048.72355794
CAAGTCG108050.026.88294
TAAGTCG34500.025.20289894
GAAGTCG135750.021.67366494
CGCGTAT4851.8189894E-1216.4742281
AAAGTCG59950.015.13094394
GTTTTTT321400.013.9069691
CGAAAAC31750.013.6188971
GTTTAAT91850.012.2808931
CGAAAAT43450.012.1150751
CGGAAAT43700.011.9382151
CGGAAAC48750.011.2799991
CGCAATT19300.011.2020731
GCGAAAT28550.011.1943961
GGAAAAT174250.011.1936871
TCGGATT25400.010.9173232
CGCGGAT26450.010.839321
CTCGGAT61800.010.7993531
CGCGAAC19050.010.6089231
CGCTAAT9054.9112714E-1110.386741
Produced by FastQC (version 0.11.5)