FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21665606
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136500.040.49230694
CAAGTCG97050.026.63575494
TAAGTCG32400.022.91975294
GAAGTCG127100.019.04405894
TATACGC10950.017.5981737
TACGCTA10700.016.2523379
GTTTTTT292550.014.8607071
GTTTAAT85500.014.2374261
CTTAGAT79100.014.2010111
ATATACG12700.014.0629926
ATTAAGC62700.014.0175446
TCAACGC42850.013.7106186
CAACGCA47350.013.4994737
CGAACGT49900.013.1863743
CGAATCG16050.013.177573
CGAAAAC39300.013.1552161
ATCAACG44550.013.081935
AAATCGA28800.013.0555554
GCGAAAT25650.012.826511
AAATCCA187400.012.7908214
Produced by FastQC (version 0.11.5)