FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22398424
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112300.055.49599594
CAAGTCG82450.027.5330594
TAAGTCG37600.023.37500294
GAAGTCG130100.022.57878594
GTTTTTT328300.014.1586961
TATACGC8400.013.9880957
AAAGTCG48250.013.73471594
TTTTTTC413850.012.34481052
CGAAAAT31950.011.9154931
CGAATCG16450.011.7142863
GTTTTAC70250.011.7081851
CGGAAAT43900.011.66970351
GTTTAAT94300.011.6129381
TACGCTA9750.011.569239
GTTTTAT170650.011.3747431
CGCAAAT36850.011.2238811
GTTTTCT235450.011.1586321
AAATCCA211200.011.0601324
GTCGATT21700.011.04608251
AAATCGA33000.010.9666674
Produced by FastQC (version 0.11.5)