FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21980976
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83850.042.3756794
CAAGTCG69950.020.6275994
TAAGTCG26000.019.70384694
GAAGTCG102850.015.85707294
GTTTTTT283250.015.0001751
TTTTTTC415000.014.1566252
CGAAAAT36250.014.1324141
AAAGTCG42350.014.0944594
CGAATCG14500.013.6137923
CGCGAAT8700.012.9655171
TTTTTTG440300.012.92686752
CGAAAAC37950.012.2608691
CGGAAAT43950.012.1911251
GTCGATT22850.012.1356671
GAAAAAT219300.012.0661191
GTTTTAC74800.012.064171
AAATCCA202450.011.9792544
ATTAAGC55450.011.8665476
GGAAAAT172700.011.8112331
GTTTTTC178500.011.7697481
Produced by FastQC (version 0.11.5)