FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21980976
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105650.033.8987294
TAAGTCG29250.020.72820394
CAAGTCG78250.018.86006494
GAAGTCG105100.015.51760194
AAATCGA35300.014.1133144
GTTTTTT283600.014.0535971
CGAAAAT45400.013.7687211
CGCATAT9750.013.0153851
CGAATCG15600.012.9551283
CGAAAAC37950.012.5085631
GGAAAAT183200.012.468341
CGCAAAC45250.012.3602211
GTTTTAT150100.012.2744841
CTCGATT39850.011.7942291
CGGAAAT43000.011.6953491
CGGATAT21100.011.5829381
AAATCCA194500.011.5506424
CGCAAAT32500.011.4246151
CTCGGAT60600.011.2458741
CGCGTTT15050.011.2425251
Produced by FastQC (version 0.11.5)